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System Biosciences Inc
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ATCC
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FUJIFILM
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Millipore
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Image Search Results
Journal: Journal of Translational Medicine
Article Title: Single-cell RNA sequencing reveals small extracellular vesicles derived from malignant cells that contribute to angiogenesis in human breast cancers
doi: 10.1186/s12967-023-04438-3
Figure Lengend Snippet: Single-cell atlas demonstrates shows association between specific endothelial and malignant cell subclusters in angiogenic breast cancer. A Venn diagram showing overlapping genes between BRCA_PPP1R1B and En_PPP1R1B. B Expression levels of common marker genes. C Gene set enrichment analysis (GSEA) showing the main pathways enriched in BRCA_PPP1R1B and En_PPP1R1B. D Effects of PPP1R1B expression on the overall survival of breast cancer patients. E Western blot analysis of PPP1R1B expression. F qRT-PCR analysis of PPP1R1B knockdown and overexpression efficiency. G Transmission electron microscope images of sEVs from T47D and MDA-MB-231 cells. Scale bar, 200 nm. H NTA showing the distribution of the size and concentration of isolated exosomes. I Western blot analysis of the expression levels of CD63, CD9, TSG101 (exosome marker genes) and calnexin (negative control) in sEVs and lysates from T47D and MDA-MB-231 cells. J Fluorescence microscope images showing the uptake of PKH26-labeled sEVs in HUVECs. Scale bar, 50 µm. K Relative expression of PPP1R1B in cells and sEVs. L Relative expression of PPP1R1B in T47D-sEVs and MDA-MB-231-sEVs after PPP1R1B knockdown and overexpression. Data were the means ± SD of three experiments. Statistical significance was determined by a two-tailed unpaired t-test ( K , L )
Article Snippet: We used the following primary antibodies: CD63 (#ab134045, abcam);
Techniques: Expressing, Marker, Western Blot, Quantitative RT-PCR, Knockdown, Over Expression, Transmission Assay, Microscopy, Concentration Assay, Isolation, Negative Control, Fluorescence, Labeling, Two Tailed Test